切换至 "中华医学电子期刊资源库"

中华乳腺病杂志(电子版) ›› 2014, Vol. 08 ›› Issue (04) : 248 -252. doi: 10.3877/cma. j. issn.1674-0807.2014.04.005

论著

血管内皮生长因子及色素上皮衍生因子在乳腺癌中的表达及临床意义
周丹1,2, 张敏3, 许鹏程3, 于洋3, 张琳3, 唐廷勇4, 吴爱国1,()   
  1. 1.510282 广州, 南方医科大学珠江医院普通外科
    2.528100 佛山,中山大学附属佛山医院,佛山市第一人民医院乳腺外科
    3.南方医科大学基础医学院组胚教研室
    4.广东医学院附属三水医院普通外科
  • 收稿日期:2014-03-17 出版日期:2014-08-01
  • 通信作者: 吴爱国
  • 基金资助:
    佛山市卫生局医学科研立项课题(2014197)

Expressions of vascular endothelial growth factor and pigment epithelium-derived factor in breast cancer and their clinical significance

Dan Zhou1, Min Zhang1, Pengcheng Xu1, Yang Yu1, Lin Zhang1, Tingyong Tang1, Aiguo Wu1,()   

  1. 1.Department of General Surgery, Zhujiang Hospital, Southern Medical University,Guangzhou 510582, China
  • Received:2014-03-17 Published:2014-08-01
  • Corresponding author: Aiguo Wu
引用本文:

周丹, 张敏, 许鹏程, 于洋, 张琳, 唐廷勇, 吴爱国. 血管内皮生长因子及色素上皮衍生因子在乳腺癌中的表达及临床意义[J/OL]. 中华乳腺病杂志(电子版), 2014, 08(04): 248-252.

Dan Zhou, Min Zhang, Pengcheng Xu, Yang Yu, Lin Zhang, Tingyong Tang, Aiguo Wu. Expressions of vascular endothelial growth factor and pigment epithelium-derived factor in breast cancer and their clinical significance[J/OL]. Chinese Journal of Breast Disease(Electronic Edition), 2014, 08(04): 248-252.

目的

探讨血管内皮生长因子(VEGF)、色素上皮衍生因子(PEDF)在乳腺癌组织中的表达水平及其与乳腺癌病理特征的关系。

方法

采用Envision 免疫组织化学方法对20 例乳腺良性病变组织(纤维腺瘤)、85 例乳腺浸润性导管癌组织中的VEGF、PEDF 和CD34 表达情况进行检测。 CD34 表达反映微血管密度(MVD)。 计数资料采用χ2 检验, Spearman 等级相关分析方法进行相关性分析。

结果

VEGF 在乳腺癌组织中的阳性表达率为71.7%(61/85),高于在乳腺良性组织中的阳性表达率40%(8/20),差异有统计学意义(χ2=9.959, P=0.002);PEDF 在乳腺癌组织中的阳性表达率为41.2%(35/85),低于在乳腺良性组织中的阳性表达率90%(18/20),差异有统计学意义(χ2 =19.683, P=0.000)。 在乳腺癌组织中VEGF 与PEDF 表达呈负相关(r=-0.365,P=0.019)。 乳腺癌组织中VEGF表达与肿瘤直径(χ2=26.31,P=0.000)、TNM 分期(χ2=5.428,P=0.020)、淋巴结转移有关(χ2=5.368,P=0.021);PEDF 表达与TNM 分期(χ2=8.584, P=0.003)、肿瘤直径(χ2=11.079,P=0.001)、绝经状态(χ2=4.507,P=0.034)、ER 状态有关(χ2 =3.974,P=0.046)。 MVD 值在PEDF 阴性组高于阳性组(38.67±6.52 比22.56±5.16,Z=-0.984,P=0.000),在VEGF 阳性组则高于阴性组(38.78±6.28 比25.36±5.12,Z=-0.972,P=0.000)。

结论

乳腺癌组织中存在PEDF、VEGF 的表达相关性,且与MVD相关,对认识乳腺癌的生物学特性以及指导乳腺癌的诊疗具有重要意义。

Objective

To investigate the expressions of vascular endothelial growth factor (VEGF)and pigment epithelium-derived factor (PEDF) in breast cancer tissue, and their relationship with the clinicopathological characteristics of breast cancer patients.

Methods

The expressions of VEGF, PEDF and CD34 were detected in 20 cases of benign breast diseases (fibroadenoma) and 85 cases of invasive ductal carcinoma by Envision immunohistochemical method. CD34 expression reflected microvessel density(MVD).The numeration data were analyzed by Chi-square test, and Spearman rank test was used for correlation analysis.

Results

The positive expression rate of VEGF was 71.7% (61/85) in breast cancer tissue,significantly higher than 40%(8/20)in breast benign tissue (χ2=9.959, P=0.002); The positive expression rate of PEDF was 41.2% (35/85)in breast cancer tissue, significantly lower than 90% (18/20)in breast benign tissue (χ2 =19.683, P=0.000); VEGF expression was negatively correlated with PEDF expression(r=-0.365, P=0.019). In invasive ductal carcinoma, the expression of VEGF was significantly related with tumor diameter (χ2=26.31,P=0.000), TNM stage (χ2=5.428,P=0.020), and lymphatic metastasis (χ2=5.368,P=0.021); the expression of PEDF was significantly related with tumor diameter (χ2 =11.079,P=0.001), TNM stage (χ2 =8.584,P=0.003), menstruation status(χ2 =4.507,P=0.034), and ER status(χ2=3.974,P=0.046). The MVD value in PEDF negative was higher than that in PEDF positive group(38.67±6.52 vs 22.56±5.16,Z=-0.984,P=0.000); The MVD value in VEGF positive was higher than that in VEGF negative group (38.78±6.28 vs 25.36±5.12,Z=-0.972,P=0.000).

Conclusion

There is a correlation between PEDF and VEGF expression in breast cancer tissue, and the expressions of the two factors are also related to MVD, which may provide reference to the physicians in recognizing the biological characteristics of breast cancer and guiding the diagnosis and treatment of breast cancer.

图1 VEGF 在乳腺浸润性导管癌中的表达( ×400) 着色位于细胞质中,呈棕黄色颗粒;VEGF:血管内皮生长因子
图2 PEDF 在乳腺浸润性导管癌中的表达( ×400) 着色位于细胞质和细胞膜中,呈棕黄色颗粒;PEDF:色素上皮衍生因子
图3 乳腺浸润性导管癌血管中CD34 阳性内皮细胞表达( ×400)
表1 乳腺癌患者的临床病理参数与PEDF、VEGF 表达情况的关系
表2 乳腺癌组织中PEDF 与VEGF 表达相关性
[1]
Jemal A, Bray F, Center MM, et al. Global cancer statistics[J]. CA Cancer J Clin,2011,61(2):69-90.
[2]
Jiménez B, Volpert OV. Mechanistic insights on the inhibition of tumor angiogenesis [J]. J Mol Med, 2001, 78 (12):663-672.
[3]
Edge SB, Compton CC. The American Joint Committee on Cancer: the 7th edition of the AJCC cancer staging manual and the future of TNM [J]. Ann Surg Oncol, 2010, 17(6):1471-1474.
[4]
郑民,梁岳培, 王洋. 非小细胞肺癌组织中VEGF、PEDF 的表达变化及意义[J]. 山东医学,2011,51(18):53-54.
[5]
Weidner N. Intratumor microvessel density as a prognostic factor in cancer[J]. Am J Pathol,1995,147(1):9-19.
[6]
Abdelhaleem M. Frequent but nonrandom expression of lymphoid markers on de novo childhood acute myeloid leukemia[J]. Exp Mol Pathol,2007,83 (2):259-263.
[7]
Thurston G, Kitajewski J. VEGF and Delta-Notch:interacting signaling pathways in tumour angiogenesis [J]. Br J Cancer,2008,99 (8):1204-1209.
[8]
Masago K, Fujita S, Kim YH, et al. Effect of vascular endothelial growth factor polymorphisms on survival in advanced-stage non-small-cell lung cancer [J]. Cancer Sci,2009,100(10):1917-1922.
[9]
Ganggaiswari A, Kresno SB, Krisnuhoni E. VEGF expression and desmoplastic reaction as potential progressive factors in young patients with colorectal cancer [J]. Acta Med Indones,2010,42 (1):6-11.
[10]
El-Sissy AH, El-Mashari MA, Bassuni WY, et al. Aberrant lymphoid antigen expression in acute myeloid leukemia in Saudi Arabia [J]. J Egypt Natl Canc lnst,2006,18 (3):244-249.
[11]
Bard MP, Hegmans JP, Hemmes A, et al. Proteomic analysis of exosomes isolated from human malignant pleural effusions[J]. Am J Respir Cell Mol Biol,2004,31(1):114-121.
[12]
Cai J, Jiang WG, Grant M, et al. Pigment epithelium-derived factor (PEDF) inhibits angiogenesis via regulated intracellular proteolysis of VEGFR1 [J]. J Biol Chem, 2006, 281 (6):3604-3613.
[13]
Offersen BV, Borre M, Overgaard J. Quantification of angiogenesis as a prognostic marker in human carcinomas: a critical evaluation of histopathological methods for estimation of vascular density [J]. Eur J Cancer,2003,39 (7):881-890.
[14]
Zhou D, Cheng SQ, Ji HF, et al. Evaluation of protein pigment epithelium-derived factor (PEDF) and microvessel density (MVD) as prognostic indicators in breast cancer[J]. J Cancer Res Clin Oncol,2010,136(11):1719-1727.
[15]
Wu W,Shu X,Hovsepyan H,et al. VEGF receptor expression and signaling in human bladder tumors [J]. Oncogene,2003,22 (22):3361-3364.
[16]
Zhang Y, Han J, Yang X, et al. Pigment epithelium-derived factor inhibits angiogenesis and growth of gastric carcinoma by down-regulation of VEGF [J]. Oncol Rep, 2011, 26(3):681-686.
[17]
Volpert OV, Zaichuk T, Zhou W, et al. Inducer-stimulated Fas targets activated endothelium for destruction by antiangiogenic thrombospondin-1 and pigment epithelium-derived factor[J]. Nat Med,2002,8 (4):349-357.
[18]
Aparicio S, Sawant S, Lara N, et al. Expression of angiogenesis factors in human umbilical vein endothelial cells and their regulation by PEDF [J]. Biochem Biophys Res Commun,2005,26(2):387-394.
[1] 李洋, 蔡金玉, 党晓智, 常婉英, 巨艳, 高毅, 宋宏萍. 基于深度学习的乳腺超声应变弹性图像生成模型的应用研究[J/OL]. 中华医学超声杂志(电子版), 2024, 21(06): 563-570.
[2] 周荷妹, 金杰, 叶建东, 夏之一, 王进进, 丁宁. 罕见成人肋骨郎格汉斯细胞组织细胞增生症被误诊为乳腺癌术后骨转移一例[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 380-383.
[3] 河北省抗癌协会乳腺癌专业委员会护理协作组. 乳腺癌中心静脉通路护理管理专家共识[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 321-329.
[4] 刘晨鹭, 刘洁, 张帆, 严彩英, 陈倩, 陈双庆. 增强MRI 影像组学特征生境分析在预测乳腺癌HER-2 表达状态中的应用[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 339-345.
[5] 张晓宇, 殷雨来, 张银旭. 阿帕替尼联合新辅助化疗对三阴性乳腺癌的疗效及预后分析[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 346-352.
[6] 邱琳, 刘锦辉, 组木热提·吐尔洪, 马悦心, 冷晓玲. 超声影像组学对致密型乳腺背景中非肿块型乳腺癌的诊断价值[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 353-360.
[7] 程燕妮, 樊菁, 肖瑶, 舒瑞, 明昊, 党晓智, 宋宏萍. 乳腺组织定位标记夹的应用与进展[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 361-365.
[8] 涂盛楠, 胡芬, 张娟, 蔡海峰, 杨俊泉. 天然植物提取物在乳腺癌治疗中的应用[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 366-370.
[9] 朱文婷, 顾鹏, 孙星. 非酒精性脂肪性肝病对乳腺癌发生发展及治疗的影响[J/OL]. 中华乳腺病杂志(电子版), 2024, 18(06): 371-375.
[10] 高杰红, 黎平平, 齐婧, 代引海. ETFA和CD34在乳腺癌中的表达及与临床病理参数和预后的关系研究[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 64-67.
[11] 韩萌萌, 冯雪园, 马宁. 乳腺癌改良根治术后桡神经损伤1例[J/OL]. 中华普外科手术学杂志(电子版), 2025, 19(01): 117-118.
[12] 张志兆, 王睿, 郜苹苹, 王成方, 王成, 齐晓伟. DNMT3B与乳腺癌预后的关系及其生物学机制[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 624-629.
[13] 王玲艳, 高春晖, 冯雪园, 崔鑫淼, 刘欢, 赵文明, 张金库. 循环肿瘤细胞在乳腺癌新辅助及术后辅助治疗中的应用[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 630-633.
[14] 赵林娟, 吕婕, 王文胜, 马德茂, 侯涛. 超声引导下染色剂标记切缘的梭柱型和圆柱型保乳区段切除术的效果研究[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 634-637.
[15] 朱佳琳, 方向, 贵诗雨, 黄丹, 周小雨, 郭文恺. 大鼠切口疝腹膜前间隙补片修补术后血清中VEGF 和Ang-1 的表达情况[J/OL]. 中华疝和腹壁外科杂志(电子版), 2024, 18(06): 703-707.
阅读次数
全文


摘要